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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which results in chromosome instability, and therefore may well lead to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important purpose in selling mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were being noted but specific nucleotide sequence information just isn't yet available.
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Blocking peptides are peptides that bind particularly towards the goal antibody and block antibody binding. These peptide generally consists of the epitope regarded with the antibody.
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This guide outlines further doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This tutorial outlines additional doable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
Antibodies bound to the blocking peptide not bind for the epitope over the concentrate on protein. By evaluating the staining from your blocked antibody as opposed to the antibody by yourself, one can see which staining is distinct.
Focus on Facts Cyclin E belongs to your really conserved cyclin family, whose members are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation patterns which add on the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and it is degraded as cells progress by click here means of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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